Fluorescence nanotomography using resonance energy transfer: demonstration with a protein-sugar complex.
نویسندگان
چکیده
A new approach to structural sensing, fluorescence resonance energy transfer nanotomography, which interprets fluorescence decay measurement in terms of site density analysis of molecular distributions, has been applied to a glucose sensor based on competitive binding with malachite green labelled dextran to the sugar binding protein concanavalin A labelled with allophycocyanin. Opportunities for structural sensing in clinical medicine are highlighted.
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ورودعنوان ژورنال:
- Physics in medicine and biology
دوره 46 9 شماره
صفحات -
تاریخ انتشار 2001